Effect of Autologus Platelet-Rich Plasma on IL-6, MMP-3 and MCP-1 Expression in Synoviocytes from Osteoarthritic Patients Knees

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Author(s)  

Chang Ich Hur¹, Cheol Park¹, Hanlou Li², Jong Keun Seon^1*, Hyun Keun Kim^1&3, Taek Rim Yoon^1&3, Eun-Kyoo Song¹

Affiliation(s)

¹Center for Joint Disease, Chonnam National University Hwasun Hospital, Jeonnam, South Korea.
²Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.
³Cardiovascular Convergence Research Center of Chonnam National University, Gwangju, South Korea.

ABSTRACT

Nowadays, some studies reported promising results of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA). However, the effects of PRP on prevention of osteoarthritis in knee joints have been debated. The present study investigated the effects of PRP on osteoarthritis related inflammatory cytokines expressed in fibroblast-like synoviocytes (FLS) from osteoarthritic knees. The synovial tissues were harvested from eight osteoarthritic patients who had undertaken total knee arthroplasties (TKAs) and cultured in DMEM containing 10% FBS. Platelet-rich plasma releasate (PRPr) was made by clotting or activation of PRP by citrate. The levels of PDGF-AA and VEGF in PRPr and whole blood were measured with ELISA method. The FLS were isolated and cultured from osteoarthritic knees. The IL-1β stimulated FLS were cultivated with three different conditions (none, 1% and 10% of PRP). To determine the expression of IL-6, MMP-3, and MCP-1, we used reverse transcriptase polymerase chain reaction (RT-PCR). The concentrations of platelet count in PRP were about 7 to 9 times higher than those of whole blood. The levels of PDGF-AA in PRPr were approximately 3 to 4 times higher than those of whole blood. The levels of VEGF in PRPr were also significantly 7 to 18 times higher than those of whole blood. Without induction of the FLS with IL-1β, 1% or 10% PRPr did not reduce expressions of inflammatory proteins (MMP-3, MCP-1), except for IL-6. However, with induction of the FLS with IL-1β, both concentrations (1% and 10%) of PRPr reduced significantly all inflammatory protein expressions (IL-6, MMP-3, MCP-1). PRPr diminished inflammatory IL-1β-mediated effects on human osteoarthritic fibroblast-like synoviocytes. These results suggest that platelet-rich plasma can be a good therapeutic option for the treatment of osteoarthritis.

KEYWORDS

Platelet-Rich Plasma, Inflammatory Cytokines, Synoviocyte, Osteoarthritis

Cite this paper

Hur, C. , Park, C. , Li, H. , Seon, J. , Kim, H. , Yoon, T. and Song, E. (2014) Effect of Autologus Platelet-Rich Plasma on IL-6, MMP-3 and MCP-1 Expression in Synoviocytes from Osteoarthritic Patients Knees. Open Journal of Regenerative Medicine, 3, 64-72. doi: 10.4236/ojrm.2014.33008. 

 

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