Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography
Read full paper at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47419#.VMirVyzQrzE Author(s) Shawn M. R. Stanley , Danny Chua Affiliation(s) The Singapore Turf Club Laboratory, 1 Turf Club Ave, Singapore, Singapore . The Singapore Turf Club Laboratory, 1 Turf Club Ave, Singapore, Singapore . ABSTRACT We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC; this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a faci...