跳至主要内容

Metabolite Profile of Lactic Acid-Fermented Soymilk

Traditional soy foods produced in Asia are known to have lipid metabolism-modulating effects. Soy protein and isoflavones are the major bioactive components in soy foods. Clinical analyses and meta-analyses have demonstrated the lipid metabolism-modulating effects of combined isoflavones and soy protein.

Soymilk is the simplest soy food, but some individuals find the flavor unpalatable. Thus, we used soymilk fermented by lactic acid bacteria of vegetable origin to make its flavor more palatable. Lactic acid fermentation converts the isoflavone glycosides in soymilk to isoflavone aglycones, enhancing their absorption in the intestine. Previous studies have shown that isoflavone aglycone-rich soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4408L (F4408) has stronger lipid metabolism-modulating effects than isoflavone aglycone-poor soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4404L (F4404). As such, fermentation products other than isoflavone aglycones may also exert lipid metabolism-modulating effects. Therefore, the present study aimed to compare the metabolites in soymilk (SM), F4404, and F4408 via a metabolomics approach.

In the study, two fermented soymilk products were prepared from soymilk via lactic acid fermentation for 15 h using Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4404L (F4404) or TUA-4408L (F4408). The soymilk metabolome analyses were carried out at Human Metabolome Technologies Inc. (HMT, Tsuruoka, Japan). Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) was carried out using an Agilent CE Capillary Electrophoresis System. Liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) was carried out using an Agilent 1200 series RRLC System SL equipped with an Agilent LC/MSD TOF (Agilent Technologies). Mass spectrometry (MS) analysis was carried out in both positive and negative ion electrospray ionization (ESI) modes of detection.

The results indicated that various aglycones including isoflavones, flavones, flavonols, and flavanones increased in both F4404 and F4408 compared with SM. The increases in isoflavone and flavanone aglycones were greater in F4408 than in F4404. Some types of dipeptides and free amino acids, especially ornithine, increased in both fermented soymilks. The increase in free amino acids, especially ornithine, was higher in F4408 than in F4404. Thus, F4408 exhibited stronger glycosidase and protease activities than F4404. Functional components produced by lactic acid fermentation are known to improve lipid metabolism.

In conclusion, it is suggested that not only isoflavone aglycones but also other functional components exert lipid metabolism-modulating effects in fermented soymilks, especially soymilk fermented using TUA-4408L.


Article by Maki Kobayashi, et al, from Japan.

Full access: http://mrw.so/4qCr3o

评论

发表评论

此博客中的热门博文

Does Immigration Promote the Investment of the Monopolistic Firm?

In the present paper, we examine the effect of increasing uncertainty of immigrants’ growth on the optimal timing of investment of a firm that has a monopolistic power over the labor market. It is revealed that when the uncertainty of immigrants’ growth is more than a threshold level, increasing uncertainty of immigrants’ growth accelerates the optimal timing of firms’ investment and enhances the economic growth, even if the uncertainty of immigrants’ growth is formulated by the geometric Brownian motion, which is in sharp contrast to the standard result that an increase in the uncertainty postpones the optimal timing. With an increase in the immigrants over the past ten years, workforces in the host countries have been growing significantly to the extent that the immigrants represent 70% of the increase in the workforce in Europe, and 47% in the United States as OECD indicates. In the present paper, we attempted to investigate the effect of increased uncertainty caused by the growi...
发表评论
阅读全文

Education Policy Implementation: A Mechanism for Enhancing Primary Education Development in Zanzibar

Education is one of the fundamental rights of individuals; therefore, the government of a country needs to develop and strengthen educational policy and quality as well as to ensure that everyone has equal access to basic education. The improvement of access and quality of education in the world is becoming as an essential factor in development, whereas the basic education (primary school), is acknowledged as a foundation of the higher educational development for every country. To fulfill this goal, governments introduce several policies and procedures; however, it requires some reforms and participation from the politician, policymakers, and other stakeholders to re-examine educational policy so that it can lead to multiplication and betterment of the reforms. Educational reforms actually focus on accountability. A positive educational development and reform is very challenging and needs more effort and strategy on how to use and utilize the resources effectively as such it can achie...
发表评论
阅读全文